ARTÍCULO
TITULO

Baseline for some plasmatic and physiological variables in Rana catesbeiana. Cien. Inv. Agr. (In English) 33(2):73-80.

José A. Coppo    
Santiago A. Fioranelli    
Pedro A. Zein    
Norma B. Mussart    

Resumen

The purpose of this study was to determine plasmatic non protein nitrogen (NPN) reference values according to sex, age, liveweight, season, and breeding and feeding systems physiological modifications in bullfrog, Rana catesbeiana.Three hundred and two healthy animals (both sexes, 9 to 21 months old) were studied. They were reared in 3 different hatcheries in northeastern Argentina. Reference values (mean ± standard deviation) were recorded for plasmatic creatinine (4.83±1.22 mg·L-1), urea (84.2±17.5 mg·L-1), and uric acid (13.4±2.89 mg·L-1). A significant correlation between growth and liveweight increase (r =0.82, P =0.02) ) was found. Significant lineal association among animal development, creatinine and urea increase, as well as uric acid decrease (P < 0.05), were registered. No significant differences were found between sexes. In amphibians fed naturally in lagoons, urea concentration was higher (105 mg·L-1) than those fed with milled lung (63.8 mg·L-1); amphibians fed with balanced pelleted diets registered intermediate values. Rearing system had a significant influence on the plasmatic NPN levels. Creatinine and uric acid lowest values were registered in amphibians coming from hatchery 3 (water covering 90% of the tank?s floor, floating feed), and highest urea and creatinine values (significant), as well as highest uric acid values (not significant), were registered in frogs reared in hatchery 1 (water covering 25% of the tank?s floor, feed supplied on dry floor). Cold season (hibernation) produced plasmatic NPN increase, significantly for urea concentration (90.1 mg·L-1 in winter versus 79.5 mg·L-1 in the remaining seasons). Utility of plasmatic NPN to evaluate sanitary, metabolic, and nutritional state of meat production frogs, is emphasized. El propósito del estudio fue obtener valores de referencia para nitrógeno no proteico (NNP) plasmático y sus variaciones fisiológicas según sexo, edad, peso, clima y sistemas de manejo y alimentación en Rana catesbeiana. Se estudiaron 302 animales sanos (ambos sexos, 9-21 meses de edad), mantenidos en tres criaderos del nordeste argentino. Se obtuvieron intervalos de referencia para creatinina (4,83±1,22 mg·L-1), urea (84,2±17,5 mg·L-1) y ácido úrico (13,4±2,89 mg·L-1). El crecimiento correlacionó con el aumento de peso (r=0,82, p=0,02). Se registró asociación lineal significativa entre el avance de la edad y los aumentos de creatinina y urea, así como con la disminución de ácido úrico (P<0,05). No se encontraron diferencias significativas entre sexos. La urea fue más alta en anfibios alimentados naturalmente en laguna (105 mg·L-1) que en animales alimentados con pulmón molido (63,8 mg·L-1). El manejo influenció significativamente los niveles de NNP. Los valores más bajos de creatinina y ácido úrico fueron registrados en anfibios del criadero Nº 3 (agua cubriendo el 90% del piso de las piletas, alimento flotante), y los valores más altos de urea y creatinina (significativos), así como de ácido úrico (no significativos), fueron registrados en el criadero Nº 1 (agua cubriendo el 25% del piso de las piletas, alimento suministrado sobre piso seco). El clima frío (hibernación) produjo aumento del NNP, significativamente para la urea (90,1 mg·L-1 en invierno versus 79,5 mg·L-1 en las estaciones restantes). Se enfatiza la utilidad del NNP para evaluar estado sanitario, metabólico y nutricional de las ranas explotadas para producción de carne.

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