ARTÍCULO
TITULO

Protocorms of an epiphytic orchid (Epidendrum amphistomum A. Richard) recovered in situ, and subsequent identification of associated mycorrhizal fungi using molecular markers

Lawrence W. Zettler    
Laura L. Corey    
Larry W. Richardson    
April Y. Ross    
Lillian Moller-Jacobs    

Resumen

Epiphytic orchids have received considerable study, yet little has been published on their germination requirements in situ involving mycorrhizal fungi. Such research has been hampered by the small, dust-like size of seeds and leafless seedlings (protocorms) which are difficult to pinpoint on natural substrates, especially those on arboreal substrates (tree limbs). We report a novel seed sowing and retrieval method, modified from one applied to terrestrial orchids, used in the acquisition of epiphytic orchid protocorms from the Florida Panther National Wildlife Refuge. Seeds from two epiphytic orchid species (Epidendrum amphistomum A. Richard, E. nocturnum Jacquin) were placed in separate nylon mesh packets secured within 35 mm plastic slide mounts, and affixed to tree bark using gutter mesh and a staple gun. To confirm that the embryos were viable, some seeds were also sown on asymbiotic media in the laboratory which subsequently germinated after 52 days incubation. Of 60 packets distributed among 18 tree limb sites, one packet - harboring seeds of E. amphistomum affixed to pop ash (Fraxinus caroliniana Mill.) on a moss substrate - harbored protocorms after 267 days. Using molecular markers, a fungus assignable to the Ceratobasidiaceae, appears to be the mycorrhizal associate of these protocorms suggesting that this fungus may be associated with the germination process in situ.

 Artículos similares

       
 
Ning Wang, Juying Jiao, Yanfeng Jia and Dongli Wang    
The Chinese Loess Plateau region has long been suffering from serious soil erosion. Thus, large-scale afforestation has continued during the past decades in order to control soil erosion. Afforestation can dramatically alter nutrient cycles, affect soil-... ver más

 
Simmi Aggarwal, C. Nirmala, Shanuja Beri, Sachin Rastogi, Alok Adholeya    
The technique of symbiotic seed germination-using fungi to cultivate orchid seedlings in vitro leading to their reintroduction in situhas considerable potential for conservation as evidenced by studies mostly in Australia and North America. However, its ... ver más

 
Tim W. Yam, Felicia Tay, Peter Ang, Weijing Soh    
Some 226 species of native orchids have been recorded in Singapore. However, of these 178 are considered to be extinct, and only five are common. The orchid conservation programme aims to monitor existing species, explore ways to conserve their germplasm... ver más

 
Lawrence W. Zettler, Erin M. Wood, Lynnaun J. A. N. Johnson, Anna K. Kirk, Steven P. Perlman    
Platanthera holochila (Hbd.) Krzl. [syn = Peristylus holochila (Hbd) N. Hallé] is the rarest of three orchids endemic to the Hawaiian Archipelago. As of 2011, 33 individual plants of this U.S. Federally endangered species remained on three islands with o... ver más

 
Morgubatul Jannat, Md. Kamruzzaman, Mohammed Kamal Hossain     Pág. 100 - 114
The study was conducted to explore natural regeneration potential of a community managed forest. Village Common Forest (VCF) has been managed as a commo n property resource under the leadership of Mouza Headmen based on traditional resource management pa... ver más