Inicio  /  Applied Sciences  /  Vol: 11 Par: 4 (2021)  /  Artículo
ARTÍCULO
TITULO

Characterization of EstDR4, a Novel Cold-Adapted Insecticides-Metabolizing Esterase from Deinococcus radiodurans

Yage Zhang    
Wei Lu    
Jin Wang    
Ming Chen    
Wei Zhang    
Min Lin    
Zhengfu Zhou and Zhu Liu    

Resumen

Cold-adapted esterases are attracting increasing attention owing to their prospective use in biotechnology. In this study, a novel cold-adapted family ? esterase EstDR4 was identified and obtained from extremophile Deinococcus radiodurans (D. radiodurans). EstDR4 displayed significant substrate preference towards short and medium chain monoesters (C2?C12). It also showed regioselectivity, enantioselectivity and degradation effects on four insecticides. The optimum temperature and pH for EstDR4 activity were 30 °C and pH 8, respectively. Additionally, EstDR4 exhibited relatively high catalytic activity at 0 °C and high stability from 10?40 °C, with over 80% of its initial activity retained after 1 h of incubation. Moreover, EstDR4 activity was stimulated by Tween 80 and Triton X-100, and inhibited by metal ions such as Co2+, Cu2+ and Zn2+ and several organic solvents. Thus, this enzyme shows development potential for many industrial biotechnological applications, including the manufacture of thermolabile pharmaceutical products, cold-wash detergents and insecticide biodegradation.

 Artículos similares

       
 
Luís P. N. Mendes, Ana M. C. Ricardo, Alexandre J. M. Bernardino and Rui M. L. Ferreira    
We present novel velocimetry algorithms based on the hybridization of correlation-based Particle Image Velocimetry (PIV) and a combination of Lucas?Kanade and Liu?Shen optical flow (OpF) methods. An efficient Aparapi/OpenCL implementation of those method... ver más
Revista: Water

 
Sungmo Kang, Hyeon Ho Shin and Zhun Li    
Microalgae are highly valued for their rapid biomass production and metabolite synthesis, as well as their abundance of beneficial compounds. They have a variety of applications, including serving as the primary ingredient in biofuels, functional foods, ... ver más

 
Verónica Herrera Gómez, Giovanni Ravazzani, Marco Mancini, Niccolò Marchi, Emanuele Lingua and Michele Ferri    
The accurate assessment of discharge in vegetated floodplains during floods is a persistent challenge in river engineering due to the difficulty of acquiring hydraulic data, the variability in vegetation roughness, and the limitations of on-site vegetati... ver más
Revista: Water

 
Ioana Demetrescu, Radu Nartita, Mihai Andrei, Andreea Cristiana Didilescu, Anisoara Cimpean and Daniela Ionita    
Amidst the prevalence of aggressive bacterial infections that can impact both oral and systemic health following various dental and implant procedures, the search for alternative, high-performing and biocompatible materials has become a challenging pursu... ver más
Revista: Applied Sciences

 
Vasile-Adrian Surdu and Romuald Gyorgy    
X-ray diffraction (XRD) is a proven, powerful technique for determining the phase composition, structure, and microstructural features of crystalline materials. The use of machine learning (ML) techniques applied to crystalline materials research has inc... ver más
Revista: Applied Sciences