Inicio  /  Applied Sciences  /  Vol: 12 Par: 1 (2022)  /  Artículo
ARTÍCULO
TITULO

Essential Oil Variability of Azorean Cryptomeriajaponica Leaves under Different Distillation Methods, Part 1: Color, Yield and Chemical Composition Analysis

Filipe Arruda    
José S. Rosa    
Ana Rodrigues    
Luísa Oliveira    
Ana Lima    
José G. Barroso and Elisabete Lima    

Resumen

This study mainly deals with the effect of hydrodistillation (HD) and water-steam distillation (WSD) methods on the color, yield, and chemical profile of the essential oil (EO) from Cryptomeria japonica fresh leaves from São Miguel Island (Azores Archipelago, Portugal). The yields of EO?HD (pale-yellowish) and EO?WSD (colorless) samples were 1.21% and 0.45% (v/w), respectively. The GC?FID, GC?MS, and 13C-NMR analyses of EO?HD vs. EO?WSD revealed (i) a high-content of monoterpenes (72.8% vs. 86.7%), mainly a-pinene (34.5% vs. 46.4%) and sabinene (20.2% vs. 11.6%), and oxygenated mono- and sesquiterpenes (20.2% vs. 9.6%); (ii) similar sesquiterpene (1.6% vs. 1.6%), ß-myrcene (5.9% vs. 5.8%), and camphene (3.5% vs. 3.8%) contents; and (iii) significant differences in other classes/components: EO?HD is richer in oxygenated sesquiterpenes (17.1%, mainly elemol (10.4%) and a-eudesmol (3.4%)) and diterpenes (3%; mostly phyllocladene), while EO?WSD is richer in oxygenated monoterpenes (7.2%, mainly terpinen-4-ol (5.4%)), p-cymene (4.4%), and limonene (3.2%). Overall, the color, yield, and quantitative composition of the EO samples studied are strongly influenced by the distillation method. Nonetheless, this C. japonica leaf EO displayed a consistent a-pinene- and sabinene-rich composition. The same chemotype was found in a commercial Azorean C. japonica leaf EO sample, obtained by industrial steam distillation (SD), as well as in Corsica C. japonica leaf EO?HD. Furthermore, the bioactive composition of our EO samples revealed the potential to be used in green plant protection and in the medical, food, cosmetic, and household industries.

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