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ARTÍCULO
TITULO

Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell walls

Rubén Polanco    
Constanza Pino    
Ximena Besoain    
Jaime Montealegre    
Luz M. Pérez    

Resumen

The secretion of enzymes involved in biocontrol from the wild T. harzianum strains Th11 and Th12 and the mutants Th11A80.1, Th11C40.1 and Th12A10.1 was studied after their cultivation in the presence of R. solani cell walls as the sole carbon source. The results showed that endoprotease activity in the supernatants from Th11A80.1 and Th11C40.1 increased 2.77 and 4.79-fold, respectively, and the ?-1,3-glucanase and ?-1,4-chitinase activity from Th12A10.1 increased 1.86 and 1.54-fold, respectively, compared with the corresponding parental strains. The role of the three enzymes in the biocontrol of R. solani is discussed.La secreción de enzimas involucradas en biocontrol por las cepas silvestres de T. harzianum Th11 y Th12, y de sus mutantes Th11A80.1, Th11C40.1 y Th12A10.1, se analizó luego de cultivarlas en medio líquido usando paredes celulares de R. solani como única fuente de carbono. Los resultados mostraron que Th11A80.1 y Th11C40.1 aumentaron la secreción de endoproteasas 2,77 y 4,79 veces, respectivamente; Th12A10.1 aumentó 1,86 veces la secreción de ?-1,3-glucanasa y 1,54 veces la de ?-1,4-quitinasa al compararlas con las cepas parentales. Se discute el rol de estas tres enzimas para el biocontrol de fitopatógenos.

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