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Inicio  /  Applied Sciences  /  Vol: 12 Par: 4 (2022)  /  Artículo
ARTÍCULO
TITULO

Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective

Daniela Correia    
Inês Domingues    
Melissa Faria and Miguel Oliveira    

Resumen

Fluoxetine is an antidepressant widely used to treat depressive and anxiety states. Due to its mode of action in the central nervous system (selective serotonin reuptake inhibitor (SSRI)), it becomes toxic to non-target organisms, leading to changes that are harmful to their survival. In this work, the effects of fluoxetine on juvenile zebrafish (Danio rerio) were evaluated, assessing biochemical (phase II biotransformation?glutathione S-transferase (GST), neurotransmission?acetylcholinesterase (ChE), energy metabolism?lactate dehydrogenase (LDH), and oxidative stress?glutathione peroxidase (GPx)) and behavior endpoints (swimming behavior, social behavior, and thigmotaxis) after 21 days exposure to 0 (control), 0.1, 1 and 10 µg/L. Biochemically, although chronic exposure did not induce significant effects on neurotransmission and energy metabolism, GPx activity was decreased after exposure to 10 µg/L of fluoxetine. At a behavioral level, exploratory and social behavior was not affected. However, changes in the swimming pattern of exposed fish were observed in light and dark periods (decreased locomotor activity). Overall, the data show that juvenile fish chronically exposed to fluoxetine may exhibit behavioral changes, affecting their ability to respond to environmental stressors and the interaction with other fish.

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