Redirigiendo al acceso original de articulo en 17 segundos...
ARTÍCULO
TITULO

Establishment and Characterization of a Skeletal Muscle-Derived Myogenic Cell Line from Black Sea Bream (Acanthopagrus schlegelii)

Dan Hee Han and Seung Pyo Gong    

Resumen

Establishing muscle lineage cell lines from fish will provide a great opportunity to study muscle development, which can eventually contribute to the improvement of the fish quality in the aquaculture industry. However, there has been a lack of the development of proper fish muscle lineage cell lines so far. Here, we report the establishment of a skeletal muscle-derived myogenic cell line from black sea bream (Acanthopagrus schlegelii). For this, we first attempted to find the optimal conditions for the primary explant culture of A. schlegelii muscle tissues and then established muscle-derived cell lines. After that, cell lines were characterized for their muscle-specific gene expression, growth, and myogenic differentiation. We found that the primary explant culture was effective when the tissue fragments were cultured in Dulbecco?s Modified Eagle Medium supplemented with fetal bovine serum and antibiotics on gelatin-coated dishes. Additionally, we confirmed that the addition of basic fibroblast growth factor was necessary to establish the cell lines. One of three cell lines established was capable of long-term culture, expressed three major myogenic regulatory genes including Pax7, MyoD, and Myog, and differentiated to myotubes in the condition using low concentration of horse serum, demonstrating that this cell line was a skeletal muscle-derived myogenic cell line.

 Artículos similares

       
 
David Castro, Pablo Estévez, José Manuel Leao-Martins, Robert W. Dickey, Natalia García-Álvarez, Fernando Real, Pedro Reis Costa and Ana Gago-Martínez    
Ciguatoxins (CTXs) are naturally occurring neurotoxins that can accumulate in fish and cause Ciguatera Poisoning (CP) in seafood consumers. Ciguatoxic fish have been detected in tropical and subtropical regions of the world including the Pacific and Indi... ver más

 
Federico Greco, Luigi Giuseppe Quarta, Aldo Carnevale, Melchiore Giganti, Rosario Francesco Grasso, Bruno Beomonte Zobel and Carlo Augusto Mallio    
Background: peritumoral collateral vessels adjacent to renal cell carcinoma (RCC) can be encountered in clinical practice. Cancer cachexia is defined as a decrease of adipose and skeletal muscle tissues. In this study we evaluated, using a quantitative C... ver más
Revista: Applied Sciences

 
Wen-Chin Ko, Chia-Ti Tsai, Kai-Cheng Hsu, Yu-Che Cheng, Tony Eight Lin, Yi-Ling Chen, Chuang-Ye Hong, Wan-Jung Lu, Chun-Ming Shih and Ting-Lin Yen    
Treatment of magnolol represents a new therapeutic strategy that may reduce the risk of vascular diseases associated with abnormal vascular smooth muscle cell (VSMC) migration or thrombin/protease-activated receptor 1 (PAR-1) activation.
Revista: Applied Sciences

 
Pierluigi Carbonara, Walter Zupa, Isabella Bitetto, Sébastien Alfonso, Mariano Dara and Matteo Cammarata    
Three groups of European sea bass (Dicentrarchus labrax) were fed for seven months, with either a conventional diet or two different organic diets, which contain organic vegetables and a natural antioxidant compound. The two organic diets differed themse... ver más

 
Flaminia Chellini, Alessia Tani, Sandra Zecchi-Orlandini, Marco Giannelli and Chiara Sassoli    
Although photobiomodulation (PBM) has proven promising to treat wounds, the lack of univocal guidelines and of a thorough understanding of light?tissue interactions hampers its mainstream adoption for wound healing promotion. This study compared murine a... ver más
Revista: Applied Sciences