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Inicio  /  Applied Sciences  /  Vol: 10 Par: 19 (2020)  /  Artículo
ARTÍCULO
TITULO

Imaging of Cell Structures Using Optimized Soft X-ray Contact Microscopy

Paulina Natalia Osuchowska    
Przemyslaw Wachulak    
Agata Nowak-Stepniowska    
Andrzej Bartnik    
Kajangi Gnanachandran    
Malgorzata Lekka    
Joanna Czwartos    
Henryk Fiedorowicz and Elzbieta Anna Trafny    

Resumen

This work is to study the relationship between the exposure conditions and the quality of cell imaging with soft X-ray contact microscopy (SXCM). It is a crucial step in the efficient visualization of cell structures. Three different human cell lines: DU145 prostate carcinoma cells, HCC38 breast cancer cells, and Poietics mesenchymal stem cells were used to establish the optimal exposure conditions in SXCM. The image quality depended on the soft X-ray (SXR) absorbed energy and photoresist development conditions. At lower SXR energy (200 or 400 SXR pulses), sharp cell edges, membrane projections, and cell?cell connections were visible. In contrast, higher energy (600 or 800 SXR pulses) allowed observation of the cytoskeleton and the nucleus in a cell type-dependent manner (the influence of cell thickness and internal complexity was noted).

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