Resumen
We use origami to create a compact, scalable three-dimensional (3-D) biobattery stack that delivers on-demand energy to the portable biosensors. Folding allows a two-dimensional (2-D) paper sheet possessing predefined functional components to form nine 3-D microbial fuel cells (MFCs), and connect them serially within a small and single unit (5.6 cm × 5.6 cm). We load the biocatalyst Pseudomonas aeruginosa PAO1 in predefined areas that form the MFCs, and freeze-dry them for long-term storage. The biobattery stack generates a maximum power and current of 20 µW and 25 µA, respectively, via microbial metabolism when the freeze-dried cells are rehydrated with readily available wastewater. This work establishes an innovative strategy to revolutionize the fabrication, storage, operation, and application of paper-based MFCs, which could potentially make energy available even in resource-limited settings.