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Inicio  /  Information  /  Vol: 15 Par: 4 (2024)  /  Artículo
ARTÍCULO
TITULO

Improving Blood Vessel Segmentation and Depth Estimation in Laser Speckle Images Using Deep Learning

Eduardo Morales-Vargas    
Hayde Peregrina-Barreto    
Rita Q. Fuentes-Aguilar    
Juan Pablo Padilla-Martinez    
Wendy Argelia Garcia-Suastegui and Julio C. Ramirez-San-Juan    

Resumen

Microvasculature analysis is an important task in the medical field due to its various applications. It has been used for the diagnosis and threat of diseases in fields such as ophthalmology, dermatology, and neurology by measuring relative blood flow or blood vessel morphological properties. However, light scattering at the periphery of the blood vessel causes a decrease in contrast around the vessel borders and an increase in the noise of the image, making the localization of blood vessels a challenging task. Therefore, this work proposes integrating known information from the experimental setup into a deep learning architecture with multiple inputs to improve the generalization of a computational model for the segmentation of blood vessels and depth estimation in a single inference step. The proposed R-UNET + ET + LA obtained an intersection over union of 0.944 ± 0.065 and 0.812 ± 0.080 in the classification task for validation (in vitro) and test sets (in vivo), respectively, and a root mean squared error of 0.0085 ± 0.0275 μ" role="presentation" style="position: relative;">µµ µ m in the depth estimation. This approach improves the generalization of current solutions by pre-training with in vitro data and adding information from the experimental setup. Additionally, the method can infer the depth of a blood vessel pixel by pixel instead of in regions as the current state of the art does.

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