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Inicio  /  Applied Sciences  /  Vol: 13 Par: 23 (2023)  /  Artículo
ARTÍCULO
TITULO

A Bilateral Craniectomy Technique for In Vivo Photoacoustic Brain Imaging

Laura S. McGuire    
Mohsin Zafar    
Rayyan Manwar    
Fady T. Charbel and Kamran Avanaki    

Resumen

Due to the high possibility of mechanical damage to the underlying tissues attached to the rat skull during a craniectomy, previously described methods for visualization of the rat brain in vivo are limited to unilateral craniotomies and small cranial windows, often measuring 4?5 mm. Here, we introduce a novel method for producing bilateral craniectomies that encompass frontal, parietal, and temporal bones via sequential thinning of the skull while preserving the dura. This procedure requires the removal of a portion of the temporalis muscle bilaterally, which adds an additional 2?3 mm exposure within the cranial opening. Therefore, while this surgery can be performed in vivo, it is strictly non-survival. By creating large, bilateral craniectomies, this methodology carries several key advantages, such as the opportunity afforded to test innovate imaging modalities that require a larger field of view and also the use of the contralateral hemisphere as a control for neurophysiological studies.