Resumen
Soil organic matter (SOM), soil aggregates, and soil microbes play key roles in agriculture soil fertility. In intercropping systems, the influences of straw incorporation and N input on the dynamics of soil physicochemical and microbial properties and their relationships are still unclear. We explore the changes in soil physicochemical and microbial properties with two straw managements, i.e., wheat straw incorporation (SI) and straw removal (SR), and four N supply rates for intercropped soybean, i.e., 60 (N60), 30 (N30), 15 (N15), and 0 (N0) kg N ha-1, in the wheat?maize?soybean relay strip intercropping systems. The results showed that SOM and SOM fractions contents, soil macroaggregate stability, and microbial and fungal a-diversity, e.g., Chao1 and Shannon indices, increased through straw incorporation and N input. The a-diversity was significantly positively correlated with soil physicochemical characteristics. Compared with SR, the relative abundance of Actinobacteria and Mortierellomycota in SI increased, but the relative abundance of Proteobacteria, Acidobacteria, and Ascomycota in SI decreased. In SI treatment, soil physicochemical characteristics and microbial diversity improved through N input, but that difference was not significant between N60 and N30. In conclusion, SI+N30 was the most effective way to maintain soil fertility and reduce the N fertilizer input in the wheat?maize?soybean relay strip intercropping.