Resumen
The use of biostimulants consisting of plant growth-promoting rhizobacteria (PGPR) has been rapidly expanding in horticulture in recent years. In the current study, a novel mix of six Bacillus sp. strains (B. subtilis, B. pumilus, B. megaterium, B. amyloliquefaciens, B. velezensis, B. licheniformis) was tested as a PGPR biostimulant in two experiments with zucchini squash (Cucurbita pepo L.). The first experiment took place in greenhouse soil in winter, while the second experiment was conducted in an open field during summer. In both experiments, seeds of the local landrace ?Kompokolokytho? and the commercial hybrid ?ARO-800? were either inoculated or non-inoculated with the PGPR biostimulant. The application of the six Bacillus sp. strains increased both the vegetative growth and the yield of zucchini squash, and these effects were associated with significantly higher shoot phosphorus levels in both experiments and both genotypes. Furthermore, at the end of the cultivation, the colony-forming units of Bacillus sp. were appreciably higher in plants originating from inoculated compared to non-inoculated seeds, indicating that the tested mix of Bacillus sp. can be successfully applied through seed inoculation. ?ARO-800? produced more vegetative and fruit biomass than ??ompokolokytho? under greenhouse cropping conditions, while in the open field crop, both genotypes performed equally. Presumably, this response occurred because ?ARO-800? did not express its full yield potential in the open field due to stress imposed by the high summer temperatures, while the local landrace, which is traditionally grown in open fields, may be more resilient to stress conditions frequently encountered in open fields.